Comparison of three different methods for the quantification of equine insulin.

BACKGROUND

precise analysis of insulin in the blood sample horse was a key element for assessing the dysregulation of insulin or insulin resistance in horses. However, previous studies have shown a marked difference in insulin concentration obtained from the analysis of samples with different immunoassays. Most of the tests used in veterinary medicine were originally  designed Rat Clia Kits for use in human diagnostics and based on antibodies directed against human insulin, although the amino acid sequences between horses and humans of different insulin. species-specific tests are used more often and seem to provide advantages compared to human-specific tests.

 The purpose of this study was to compare the three immunoassays, one pig-specific insulin enzyme-linked immunosorbent assay (ELISA), advertised to be specific for insulin horse, a pig specific radioimmunoassay insulin (RIA) and the human-specific immunoassay chemiluminescence insulin (CLIA) three are widely used in animal laboratory for equine insulin analysis. In addition, we test the clinical application in assessing insulin resistance and dysregulation with blood analysis and basal blood samples obtained during diagnostic dynamic stimulation test (OGT) with increased insulin concentration.

RESULTS

insulin value obtained from ELISA, RIA and CLIA, investigated for basal blood sample analysis differed significantly between the three tests. Analysis of samples obtained during diagnostic testing dynamic stimulation with high insulin concentration in sequence revealed a significantly (p <0.001) lower insulin concentrations provided by CLIA compared to ELISA. However, the values ​​measured by ELISA were intermediate and not different from that measured by RIA. Calculated recovery after dilution, as a marker to test the accuracy of the diluted sample, was 98 ± 4% for the ELISA, 160 ± 41% for the RIA and 101 ± 11% for CLIA.

CONCLUSION

Our results indicate that insulin concentrations of the samples was measured by different methods vary widely and should be interpreted with caution. Consideration of immunoassay methods and specific assay reliable reference ranges are of particular importance, especially in clinical cases where small changes in insulin levels can lead to a false classification in terms of insulin sensitivity horses and ponies. 

CASE 7 years description of female sexual intact snow leopard (Panthera uncia) is checked for blepharospasm, debit periocular, ventral deviation of cilia upper eyelid, prolapse of the third eyelid, and the opacity of the cornea of ​​the right eye. CLINICAL FINDINGS eye examinations performed with the patient sedated ulcer revealed a 3 × 3-mm extended about 60% of the corneal depth right and was accompanied by a cellular infiltrate perilesional and intralesional and active vascularization.

 Upper eyelid of the right eye also has previous coloboma resulting in Simian Clia Kits trichiasis corrected. TREATMENT AND RESULTS Surgical intervention was elected after five weeks of medical management including topical administration of autologous serum and topical, subconjunctival and systemic administration of antimicrobial failed to produce an improvement in the ulcer.